GREAT AT SMALL THINGS

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LMG media details

Last data update: 24 January 2024 16:39 CET  

1 medium found

Name: Medium for Fimbriiglobus ruber

Medium number: 1038

Recipe: 0.1 g KH2PO4

20 ml Mineral salt sol. (Hutner/Basal Salt)

1 g N-acetylglucosamine

0.5 g glucose

0,05 g peptone

0.1 g yeast extract

pH 5.8-6.2



Mineral salt sol. (Hutner/Basal Salt)

Mineral salt sol. (Hutner/Basal Salt):

NTA 10 g

MgSO4 x 7H2O 29,70 g

CaCl2 x 2H2O 3,34 g

Na2MoO4 x 2H2O 12,67 mg

FeSO4 x 7H2O 99 mg

Metall salt sol. "44" 50 ml

Distilled water add to 1L

Dissolve NTA in 700 ml distill water by adjusting pH to 7.2 with KOH. Dissolve all components separately and combine slowly. Sterilize by filtration. Store at 4°C



"Metals 44":

Na-EDTA 250 mg

ZnSO4 x 7H2O 1095 mg

FeSO4 x 7H2O 500 mg

MnSO4 x H2O 154 mg

CuSO4 x 5H2O 39,5 mg

CoCl2 x 6H2O 20,3 mg

Na2B4O7 x 10H2O 17,7 mg

Distilled water 1L

Dissolve the EDTA in 700 ml and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metals if necessary. Store at 4°C. Do not autoclave.