Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | Modified NMS medium for Methyloprofundus sedimenti |
| Medium number: | 1039 |
| Recipe: | Solution 1: NMS salt stock: KNO3 1,0 g MgSO4 x 7H2O 1,0 g CaCl2 x 6H2O 0,2 g Distilled water 1 L Solution 2: Phosphate buffer Na2HPO4 x 12H2O 14,34 g KH2PO4 5,44 g Distilled water 1 L pH = 6,8 Solution 3 : Trace element sol. (1000x): Nitrilotriacetic acid (NTA) 1,5 g MgSO4 x 7H2O 3,0 g MnSO4 x H2O 0,5 g NaCl 1,0 g FeSO4 x 7H2O 0,1 g CoSO4 x 7H2O 0,18 g CaCl2 x 2H2O 0,1 g ZnSO4 x 7H2O 0,18 g CuSO4 x 5H2O 0,01 g KAI(SO4)2 x 12H2O 0,02 g H3BO3 0,01 g Na2MoO4 x 2H2O 0,01 g NiCl2 x 6H2O 0,03 g Na2SeO3 x 5H20 0,3 mg Distilled water 1000 ml Dissolve NTA first and adjust pH to 6,5 with KOH (~0,5g for 350 ml), then add other salts. Adjust pH to 7.0 with KOH. Filter sterilize. Solution 4: Vitamin sol. (1000x.): Biotin 2,0 mg Folic acid 2,0 mg Pyridoxine-HCl 10,0 mg Thiamine-HCl x 2 H2O 5,0 mg Riboflavin 5,0 mg Nicotinamide 5,0 mg D-Ca-pantothenate 5,0 mg Vitamin B12 0,1 mg p-Aminobenzoic acid 5,0 mg Lipoic acid 5,0 mg Distilled water 1 L Filter sterilize. Solution 5: Add 300 g NaCl in 1 L distilled water. Autoclave Solution 6: Add 38 g Fe-EDTA in 1 L distilled water. Filter sterilize To make 1 L liquid NMS add 100 ml Solution 1 50 ml Solution 2 1 ml Solution 3 1 ml Solution 4 66 ml Solution 5 400 µl Solution 6 Bring to 1 L with sterile H2O |