GREAT AT SMALL THINGS

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LMG media details

Last data update: 16 April 2021 10:30 CEST  

1 medium found

Name:

Medium number: 104

Recipe: Solution A:

K2HPO4 0,5 g

NH4Cl 1 g

Na2SO4 1 g

CaCl2 x 2H2O 0,1 g

MgSO4 x 7H2O 2 g

DL-Sodium lactate 2 g

Yeast extract 1 g

Resazurin 1 mg

Distilled water 980 ml

pH 7,4

Solution B:

FeSO4 x 7H2O 0,5 g

Distilled water 10 ml

Solution C:

Sodium thioglycolate 0,1 g

Ascorbic acid 0,1 g

Distilled water 10 ml

Combine the three solutions and adjust pH to 7,8 before autoclaving. If needed, add 15 g agar per liter medium.