Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | 3.8M CM1 |
| Medium number: | 1063 |
| Recipe: | 3.8M CM1 (To prepare 1L) 829mL of 30% Salt Water (see below) 5mL of 23% MGM (see below) 55mL of deionized water 100mL of 250mM pyruvate After autoclaving add the following from sterile stocks: 5mL 1M NH4Cl 2mL 0.5M K2HPO4 0.5mL 10X AML60 Solution C (see below) 0.5mL 10X AML60 Solution D (see below) 3mL 1000X Vitamin Solution (see below) 30% Salt Water (1L): 240g NaCl 24.87g MgCl2 * 6H2O 29.015g MgSO4*7H2O Dissolve salts completely then add 5mL CaCl2*H2O from a sterile 1M stock. Adjust pH to 7.5 23% MGM (100mL) 76.7mL 30% Salt Water 20mL deionized water 0.5g peptone (Don't use Difco Bacto-peptone, it was reported in 1988 to contain bile salts that lyse halobacteria, this was still the case in 2001) 0.1g yeast extract Adjust pH to 7.5 AML60 Solution C (amounts are g/L for a 10X solution) 1.9g CoCl2*6H2O 1.0g MnCl2*4H2O 0.7g ZnCl2 0.06g H3BO3 0.36g Na2MoO4*2H2O 0.36g NiCl2*6H2O 0.02g CuCl2*H2O AML60 Solution D (amounts are g/L for a 10X solution) 1.5g Ferrous Sulfate 99mL deionized water 1mL 1N sulfuric acid Vitamin Solution (1L in H2O) 13mg p-aminobenzoic acid 33mg nicotinic acid 17mg hemicalcium D-(+)-pantothenate 33mg thiamine hydrochloride 17mg cyanocobalamin 10mg riboflavin |