GREAT AT SMALL THINGS

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LMG media details

Last data update: 20 October 2021 09:31 CEST  

1 medium found

Name: Anaerobic freshwater medium

Medium number: 1111

Recipe: solution A 930 ml

solution B 1 ml

solution C 1 ml

solution D 1 ml

solution E 50 ml

solution F 10 ml

solution G 10 ml



solution A

Na2SO4 0.15 g

NaNO3 0.7 g

KH2PO4 0.20 g

NH4Cl 0.25 g

NaCl 1.00 g;

MgCl2 x 6H2O 0.40 g

KCl 0.50 g

CaCl2 x 2H2O 0.15 g

Distilled water 920 ml



Solution B (Trace element solution SL-10)

Hydrochloric acid (25%) 10 ml

FeCl2 x 4H2O 1.5 g

CoCl2 x 6H2O 0.190 g

MnCl2 x 2H2O 0.100 g

ZnCl2 0.07 g

NiCl2 x 6H2O 0.024 g

Na2MoO4 x 2H2O 0.036 g

H3BO3 0.006 g

CuCl2 x 2H2O 0.002 g

Distilled water 1L



Solution C

Vit B12 100 mg

p-aminobenzoic acid 80 mg

D(+)-Biotin 20 mg

Nicotinic acid 200 mg

Calcium pantothenate 100 mg

Pyridoxine Hydrochloride 300 mg

Thiamine-HCM x 2H2O 200 mg

Distilled water 1L



Solution D

NaOH 0.5 g

Na2SeO3 x 5H2O 3 mg

Na2WO4 x 2H2O 4 mg

Distilled water 1L



Solution E

NaHCO3 2.5g

Distilled water 50 ml



Solution F

Resorcinol 0.22 g

Distilled water 10ml



Solution G

Na2S x 9H2O 0.3 g

Distilled water 10 ml