GREAT AT SMALL THINGS

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LMG media details

Last data update: 21 October 2021 10:13 CEST  

1 medium found

Name: Modified MRS medium

Medium number: 1115

Recipe: Tryptone 10 g

Beef extract 5 g

Yeast extract 5 g

Sodium acetate x 3H2O 5 g

Ammonium citrate 2 g

K2HPO4 x 3H2O 2,6 g

MgSO4 x 7H2O 0,1 g

MnSO4 x 4H2O 50 mg

Cysteine HCl 0,5 g

Tween 80 1 g

Agar 15 g

Distilled water 900 ml

Adjust medium for final pH 6.3. Autoclave and cool to approx. 50°C. Aseptically add the following filter-sterilized solution :

Glucose 7 g

Fructose 7 g

Maltose 7 g

Sodium gluconate 2 g

Distilled water 100 ml