GREAT AT SMALL THINGS

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LMG media details

Last data update: 23 June 2021 10:40 CEST  

1 medium found

Name:

Medium number: 115

Recipe: Yeast extract 10 g

Activated charcoal (Norit SG) 2 g

ACES buffer (Sigma) 10 g

Agar 17 g

Hydrate ACES in 500 ml distilled water at 50°C, then mix with a solution containing 40 ml of 1 N KOH in 440 ml distilled water. Use the mixture to hydrate the other ingredients. Autoclave for 15 minutes. After sterilization, cool to 50°C, and add the following filter-sterilized solution:

Cysteine hydrochloride 0,4 g

Fe4(P2O7)3 0,25 g

Distilled water 20 ml

The final pH of the medium should be 6,9.