GREAT AT SMALL THINGS

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LMG media details

Last data update: 09 August 2022 13:48 CEST  

1 medium found

Name:

Medium number: 141

Recipe: CaCL2 x 2H2O 0,25 g

MgSO4 x 7H2O 0,5 g

(NH4)2SO4 0,2 g

K2HPO4 3 g

Yeast extract 2 g

Glucose 5 g

Trace Elements 1 ml

(see below)

Agar 15 g

Distilled water 1 L

Trace Elements:

CaCl2 x 2H2O 0,66 g

ZnSO4 x 7H2O 0,18 g

CuSO4 x 5H2O 0,16 g

MnSO4 x 4H2O 0,15 g

CoCl2 x 6H2O 0,18 g

H3BO3 0,1 g

Na2MoO4 x 2H2O 0,3 g

Distilled water 1 L

Adjust medium for final pH 4,0 with H2SO4. Autoclave agar separately to avoid hydrolysis. Autoclave medium at 121°C for 15 minutes.