Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | |
| Medium number: | 174 |
| Recipe: | Solution A CaCl2 x 2H2O 0,25 g MgSO4 x 7H2O 0,5 g (NH4)2SO4 0,2 g K2HPO4 3 g Yeast extract 2 g Glucose 5 g Distilled water for liquid medium: 1000 ml for solid medium: 500 ml Adjust to pH 4.0 with H2SO4 SL-6 Trace Elements Solution: ZnSO4 x 7H2O 0,1 g MnCl2 x 4H2O 0,03 g H3BO3 0,3 g CoCl2 x 6H2O 0,2 g CuCl2 x 2H2O 0,01 g NiCl2 x 6H2O 0,02 g Na2MoO4 x H2O 0,03 g Distilled water 1 L Adjust pH of Trace Elements Solution to 3,4. Solution B : Agar 15 g Distilled water: 500 ml Autoclave solutions separately to avoid hydrolysis of the agar. Autoclave at 121°C for 15 minutes. For liquid medium combine solution A (with 1000 ml distilled water) and solution SL-6 (1 ml). For solid medium combine solution A (with 500 ml distilled water), solution SL-6 (1 ml) and solution B (500 ml). For strains of Alicyclobacillus cycloheptanicus add 5g/l yeast extract instead of 2 g/l. |