GREAT AT SMALL THINGS

0

LMG media details

Last data update: 24 January 2024 16:39 CET  

1 medium found

Name: Azoarcus SM medium

Medium number: 202

Recipe: Solution A:

Neutralize in bidistilled water 5 g malic acid with KOH (approximate amount of 4,5 g). Check pH. Adjust if necessary.

Supplement with :

MgSO4 x 7H2O 0,2 g

NaCl 0,1 g

CaCl2 20 mg

Na2MoO4 x 2H2O 2 mg

MnSO4 x H2O 10 mg

Fe EDTA (0,66% w/v in H2O) 10 ml

Adjust to a final volume of 1 L with bidistilled water taking into account the volume of solution B to be added. Autoclave.

Solution B:

Mix 0,4 g K2HPO4 and 0,6 g KH2PO4 in bidistilled water and check pH. Adjust if necessary. Autoclave separately.

Aseptically mix solution B with solution A. Final pH is 6,8.