GREAT AT SMALL THINGS

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LMG media details

Last data update: 26 March 2021 09:07 CET  

1 medium found

Name: Sea water agar

Medium number: 21

Recipe: 'Lab-Lemco' beef extract 10 g

Peptone 10 g

Agar 15 g

Filtered, aged seawater * 750 ml

Tap water 250 ml

Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Cool to 50°C and add warm (50°C) sterile sea water.

* If natural sea water is not available, use artificial sea water :

NaCl 28,13 g

KCl 0,7 7 g

CaCl2 1,20 g

MgCl 2 2,55 g

NaHCO 3 0,11 g

MgSO4 x 7H2O 3,50 g

Distilled water 1 L