Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | |
| Medium number: | 274 |
| Recipe: | Agar base: Agar 7,5 g Distilled water 250 ml Solution A: Yeast extract (Oxoid) 25 g Distilled water 100 ml Solution B: Glucose 25 g Distilled water 100 ml Sterilize the agar base by autoclaving and cool to 60°C. Sterilize solution A and solution B by filtration. Aseptically add 10 ml of A and 10 ml of B to 250 ml low-fat UHT milk. Mix well and add very slowly 2 ml of 20% glacial acetic acid while shaking. Adjust pH to 5,4. Aseptically add 250 ml of the agar base solution. Shake and immediately poor into plates. |