LMG media details

Last data update: 22 June 2021 11:35 CEST  

1 medium found


Medium number: 28

Recipe: Agar base:

(NH4)2SO4 2 g

KH2PO4 1 g

K2HPO4 2 g

MgSO4 x 7H2O 0,3 g

NaCl 20 g

Agar 15 g

Distilled water 1 L

Solution A:

FeSO4 x 7H2O 28 mg

Na2MoO4 x 2H2O 24 mg

MnSO4 x H2O 76 mg

CaCl2 x 2H2O 15 mg

ZnSO4 x 7H2O 0,14 g

CoCl2 x 6H2O 24 mg

CuSO4 x 5H2O 25 mg

Distilled water 100 ml

Solution B:

Vitamin B12 0,1 mg

Distilled water 100 ml

Add 1 ml of solution A per liter agar base. Adjust pH to 7,0 and sterilize. Allow to cool to about 45°C before adding 1 ml of filter-sterilized solution B and 10 ml filter-sterilized methanol per liter medium.