Last data update:
24 January 2024 16:39 CET
1 medium found
Name: | |
Medium number: | 283 |
Recipe: | Supplement 1 L of medium 115 with 0,1% alpha-ketoglutarate. Medium 115 Yeast extract 10 g Activated charcoal (Norit SG) 2 g ACES buffer (Sigma) 10 g Agar 17 g Hydrate ACES in 500 ml distilled water at 50°C, then mix with a solution containing 40 ml of 1 N KOH in 440 ml distilled water. Use the mixture to hydrate the other ingredients. Autoclave for 15 minutes. After sterilization, cool to 50°C, and add the following filter-sterilized solution: Cysteine hydrochloride 0,4 g |