Last data update:
24 January 2024 16:39 CET
1 medium found
Name: | |
Medium number: | 296 |
Recipe: | K2HPO4 0,8 g KH2PO4 0,2 g MgSO4 x 7H2O 0,5 g FeSO4 x 7H2O 0,01 g (NH4)2SO4 1 g Trace element solution 1 ml see below Vitamin solution 5 ml see below Agar 15 g Distilled water 1 L Add 500 µl of hexane (C-source) into the lid of a Petri-dish containg this medium. The strain must grow in the vapour without direct contact with the cells. Trace element solution: Na2 EDTA 3 g MnCl2 x 2H2O 0,05 g CoCl2 x 6H2O 0,19 g ZnCl2 0,041 g H3BO3 0,006 g NiCl2 x 6H2O 0,024 g CuCl2 0,002 g Na2MoO4 x 2H2O 0,018 g Distilled water 1 L Adjust pH to 6. Vitamin solution: Thiamine 0,01 g Nicotinic acid 0,02 g Pyridoxine hydrochloride 0,02 g p -Aminobenzoic acid 0,01 g Riboflavin 0,02 g Panthotein acid 0,02 g Biotin 0,001 g Cyanocobalamin 0,001 g Distilled water 1 L Adjust pH to 7. |