GREAT AT SMALL THINGS

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LMG media details

Last data update: 17 September 2021 10:55 CEST  

1 medium found

Name: ABM2 (Alkaline Buffered medium 2)

Medium number: 316

Recipe: Yeast extract 5 g

Triptone 5 g

Alpha-ketoglutaric acid

monopotassium salt (Sigma) 1 g

Macronutrients

solution 10x 100 ml

Micronutrients

solution 100x 10 ml

Carbonate-bicarbonate buffer

solution 1 M pH 9 100 ml

Agar 15 g

Distilled water up to 1 L

Adjust pH to 9 with NaOH.

Autoclave at 121°C for 15 minutes.

Macronutrients solution 10x:

Nitrilotriacetic acid 1 g

CaSO4 x 2H2O 0,6 g

MgSO4 x 7H2O 1 g

NaCl 0,8 g

KNO3 1,03 g

NaNO3 6,89 g

Na2HPO4 1,11 g

Distilled water 1 L

Micronutrients solution 100x:

MnSO4 x H2O 220 mg

ZnSO4 x 7H2O 50 mg

H3BO3 50 mg

CuSO4 x 5H2O 2,5 mg

Na2MoO4 x 2H2O 2,5 mg

CoCl2 x 6H2O 4,6 mg

Distilled water 1 L