GREAT AT SMALL THINGS

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LMG media details

Last data update: 24 October 2021 17:13 CEST  

1 medium found

Name: Middelbrook medium

Medium number: 336

Recipe: Bacto Middelbrook 7H10 agar 19 g (see below)

Glycerol 5 mL

Distilled water 900 mL

Sterilize for 10 min by 121°C, cool to 50-55°C. Add 100 ml Bacto Middelbrook OADC enrichment Difco(see below) under aspetic conditions and mix thoroughly.

Bacto Middelbrook 7H10 agar:

(NH4) SO4 0,5 g

KH2PO4 1,5 g

Na2HPO4 1,5 g

Na-citrate 0,4 g

MgSO4 0,025 g

CaCl2 0,5 mg

ZnSO4 1 mg

CuSO4 1 mg

L-Glutamic acid 0,5 g

Ferric ammonium citrate 0,04 g

Pyridoxine 1 mg

Biotin 0,5 mg

Malachite green 0,25 mg

Bacto agar 15 g

Distilled water 1 L

pH 6,6

Bacto Middelbrook OADC enrichment:

Oleic acid 0,05 g

Albumin fraction V, bovine 5 g

Dextrose 2 g

Catalase (beef) 4 mg

NaCl 0,85 g

Distilled water 100 ml