Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | Middelbrook medium |
| Medium number: | 336 |
| Recipe: | Bacto Middelbrook 7H10 agar 19 g (see below) Glycerol 5 mL Distilled water 900 mL Sterilize for 10 min by 121°C, cool to 50-55°C. Add 100 ml Bacto Middelbrook OADC enrichment Difco(see below) under aspetic conditions and mix thoroughly. Bacto Middelbrook 7H10 agar: (NH4) SO4 0,5 g KH2PO4 1,5 g Na2HPO4 1,5 g Na-citrate 0,4 g MgSO4 0,025 g CaCl2 0,5 mg ZnSO4 1 mg CuSO4 1 mg L-Glutamic acid 0,5 g Ferric ammonium citrate 0,04 g Pyridoxine 1 mg Biotin 0,5 mg Malachite green 0,25 mg Bacto agar 15 g Distilled water 1 L pH 6,6 Bacto Middelbrook OADC enrichment: Oleic acid 0,05 g Albumin fraction V, bovine 5 g Dextrose 2 g Catalase (beef) 4 mg NaCl 0,85 g Distilled water 100 ml |