Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | Saltwater medium |
| Medium number: | 385 |
| Recipe: | NaCl 24,32 g MgCl2 x 6H2O 10 g CaCl2 x 2H2O 1,5 g KCl 0,66 g Na2SO4 4 g HEPES 2,38 g Peptone 0,2 g Yeast extract 0,1 g KBr 840 µM H3BO3 400 µM SrCl2 150 µM NH4Cl 400 µM KH2PO4 40 µM NaF 70 µM SL10 (see below) 1 ml Distilled water 1 L Adjust pH to 7,5 -8.0 with NaOH After autoclaving, let cool to 55°C, add: - 1 ml 5-fold-vitamins-solution (see below) - 15 ml 1 M NaHCO3 (autoclaved separately) SL10 Hydrochloric acid (25%) 10 ml FeCl2 x 4H2O 1,5 g CoCl2 x 6H2O 0,190 g MnCl2 x 2H2O 0,100 g ZnCl2 0,070 g NiCl2 x 6H2O 0,024 g Na2MoO4 x 2H2O 0,036 g H3BO3 0,006 g CuCl2 x 2H2O 0,002 g Distilled water 1000 ml First solve FeCl2 in hydrochloric acid, add some water and the other components, adjust to 1L. 5-fold-vitamins-solution Biotin 10 mg Folic acid 10 mg Pyridoxinhydrochloride 50 mg Thiaminhydrochloride 25 mg Riboflavin 25 mg Nicotinic acid 25 mg DL-calcium pantothenate 25 mg Vitamin B12 0,5 mg p-aminobenzoic acid 25 mg Lipoic acid 25 mg Distilled water 1000 ml Filter sterilize |