Last data update:
24 January 2024 16:39 CET
1 medium found
Name: | DNMS medium |
Medium number: | 391 |
Recipe: | dNMS Salt Stock: MgSO4 x 7H2O 10,0 g KNO3 10,0 g CaCl2 x 2H2O 1,5 g Distilled water 1 L FeNaEDTA Stock FeNaEDTA 0,5 g Distilled water 100 ml Na2HPO4 Stock Na2HPO4 x 12H2O 71,7 g Distilled water 1 L KH2PO4 Stock KH2PO4 27,2 g Distilled water 1 L 1000x trace solution: Na2EDTA x 2H2O 0,5 g FeSO4 x 7H2O 0,2 g H3BO3 0,03 g CoCl2 x 6H2O 0,02 g ZnSO4 x 7H2O 0,01 g MnCl2 x 4H2O 0,003 g Na2MoO4 x 2H2O 0,003 g NiCl2 x 6H2O 0,002 g CuSO4 x 5H2O 2,5 g Distilled water 1 L Dilute 20ml of DNMS salts stock to 800ml. Add 1 ml FeNaEDTA Stock and 1 ml trace solution. Dissolve and bring to 1 liter (distilled water) For solid media add 15g/l Bacto Agar. After sterilization, cool to 50°C-60°C and add first 5ml KH2PO4 Stock (filter sterilized) followed by 5ml Na2HPO4 Stock (filter sterilized). pH of the medium should be 6,8 |