Last data update:
24 January 2024 16:39 CET
1 medium found
Name: | ONR7a minimal medium with acetate and marine supplements |
Medium number: | 396 |
Recipe: | 5M NaCl 28 ml 10% Na-acetate solution 10 ml Solution A 100 ml Solution B 100 ml Solution C 100 ml Bactoagar 15 g Distilled water 670 ml Solution A: NaCl 146 g Na2SO4 39,8 g TAPSO 13 g KCl 7,2 g NH4Cl 2,7 g Na2HPO4 x 7H2O 0,89 g NaBr 0,83 g NaHCO3 0,31 g H3BO3 0,27 g NaF 26 mg Distilled water 1 L Adjust pH to 7,9 Solution B: MgCl2 x 6H2O 55,9 g CaCl2 7,3 g SrCl2 x 6H2O 0,12 g Distilled water 500 ml Solution C: FeCl3 x 6H2O 11,8 mg Distilled water 500 ml After sterilization, add 5ml of the filter sterilized Marine Supplements (100x) solution: Marine Supplements (100x): Trace Elements (10x) 1 ml Distilled water (sterile) 94 ml Vitamin Mixture (2x) 5 ml Trace Elements (10x) Na2EDTA 4,36 g FeCl3 x 6H2O 3,15 g ZnSO4 x 7H2O 0,022 g CoCl2 x 6H2O 0,01 g MnCl2 x 4H2O 0,18 g Na2MoO4 x 2H2O 0,0063 g Distilled water 100 ml Vitamin Mixture (2x) Biotin 0,0005 g Thiamine hydrochloride 0,1 g Cyanocobalamin 0,0005 g Distilled water 100 ml |