GREAT AT SMALL THINGS

0

LMG media details

Last data update: 21 October 2021 10:13 CEST  

1 medium found

Name: ONR7a minimal medium with acetate and marine supplements

Medium number: 396

Recipe: 5M NaCl 28 ml

10% Na-acetate solution 10 ml

Solution A 100 ml

Solution B 100 ml

Solution C 100 ml

Bactoagar 15 g

Distilled water 670 ml

Solution A:

NaCl 146 g

Na2SO4 39,8 g

TAPSO 13 g

KCl 7,2 g

NH4Cl 2,7 g

Na2HPO4 x 7H2O 0,89 g

NaBr 0,83 g

NaHCO3 0,31 g

H3BO3 0,27 g

NaF 26 mg

Distilled water 1 L

Adjust pH to 7,9

Solution B:

MgCl2 x 6H2O 55,9 g

CaCl2 7,3 g

SrCl2 x 6H2O 0,12 g

Distilled water 500 ml

Solution C:

FeCl3 x 6H2O 11,8 mg

Distilled water 500 ml

After sterilization, add 5ml of the filter sterilized Marine Supplements (100x) solution:

Marine Supplements (100x):

Trace Elements (10x) 1 ml

Distilled water (sterile) 94 ml

Vitamin Mixture (2x) 5 ml

Trace Elements (10x)

Na2EDTA 4,36 g

FeCl3 x 6H2O 3,15 g

ZnSO4 x 7H2O 0,022 g

CoCl2 x 6H2O 0,01 g

MnCl2 x 4H2O 0,18 g

Na2MoO4 x 2H2O 0,0063 g

Distilled water 100 ml

Vitamin Mixture (2x)

Biotin 0,0005 g

Thiamine hydrochloride 0,1 g

Cyanocobalamin 0,0005 g

Distilled water 100 ml