Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | Nitrate Mineral Salts (NMS) medium |
| Medium number: | 399 |
| Recipe: | 10x NMS salt stock: KNO3 10,0 g MgSO4 x 6H2O 10,0 g CaCl2 x 2H2O 2 g Distilled water 1 L FeEDTA stock FeEDTA 3,8 g Distilled water 100 ml Sodium molybdate stock Na2MoO4 x 2H2O 0,26 g Distilled water 1 L Trace solution: CuSO4 x 5H2O 1,0 g FeSO4 x 7H2O 2,5 g ZnSO4 x 7H2O 2,0 g H3BO3 0,075 g CoCl2 x 6H2O 0,250 g Na2EDTA 1,250 g MnCl2 x 4H2O 0,1 g NiCl2 x 6H2O 0,05 g Distilled water 5 L Phosphate buffer Na2HPO4 x 12H2O 71,6 g KH2PO4 26,0 g Distilled water 1 L pH = 6,8 Dilute 100ml of 10x NMS salts stock to 1L. Add 1 ml of sodium molybdate stock and 1 ml of the trace solution. Add 0,1 ml of FeEDTA Stock. Add 15g/l Bacto agar.for plates. After sterilization, cool enough to hold in the hand and aseptically add 10 ml phosphate buffer (autoclaved separately). If this is done too early the phosphate will precipitate out. |