Last data update:
27 September 2023 10:19 CEST
1 medium found
| Name: | SSE/HD1:10, pH 5,5 |
| Medium number: | 406 |
| Recipe: | Solution A: Peptone 0,5 g Yeast extract 0,25 g Glucose 0,1 g MES (10mM concentration) 1,95 g SSE (2x) (see below) 500 ml Adjust pH to 5,5 autoclave Solution B: Agar (purified) 15 g Distilled water 500 ml Do not adjust pH, autoclave After autoclaving, let cool solution A and B to 55°C, combine and add 1ml SL10 and 1 ml 10-vitamins-solution (100x) (see below) SSE (2x) CaCl2 x 2H2O 0,2938 g NH4Cl 0,1069 g MgCl2 x 6H2O 0,2036 g (NH4) 2SO4 0,1983 g MgSO4 x 7H2O 0,7390 g CaSO4 x 2H2O 0,8606 g Ca(NO3)2 x 4H2O 0,2360 g NaNO3 0,4240 g KH2PO4 (100mM solution) 0,5 ml FeSO4 x 7H2O 0,0111 g K2SO4 0,0870 g Distilled water 1000 ml Stir for minimum 5h and autoclave in case of no direct use (storage). SL10 Hydrochloric acid (25%) 10 ml FeCl2 x 4H2O 1,5 g CoCl2 x 6H2O 0,190 g MnCl2 x 2H2O 0,100 g ZnCl2 0,070 g NiCl2 x 6H2O 0,024 g Na2MoO4 x 2H2O 0,036 g H3BO3 0,006 g CuCl2 x 2H2O 0,002 g Distilled water 1000 ml First solve FeCl2 in hydrochloric acid, add some water and the other components, ajust to 1L. Do not adjust pH (is about 1,15), filter sterilize. 10-vitamins-solution (100x) Biotin 2 mg Folic acid 2 mg Pyridoxinhydrochloride 10 mg Thiaminhydrochloride 5 mg Riboflavin 5 mg Nicotinic acid 5 mg DL-calcium pantothenate 5 mg Vitamin B12 0,1 mg p-aminobenzoic acid 5 mg Lipoic acid 5 mg Distilled water 1000 ml Filter sterilize |