GREAT AT SMALL THINGS

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LMG media details

Last data update: 24 January 2024 16:39 CET  

1 medium found

Name: Modified 337a medium

Medium number: 416

Recipe: Solution A:

KH2PO4 1,3 g

Na2HPO4 1,13 g

(NH4)2SO4 0,5 g

MgSO4 x 7H2O 0,2 g

CaCl2 x 2H2O 3,09 mg

FeSO4 x 7H2O 2,0 mg

Na2MoO4 x 2H2O 1,0 mg

MnSO4 x 4H2O 0,88 mg

Distilled water 500 ml

Adjust pH to 7,5



Solution B:

Agar (purified) 15 g

Distilled water 500 ml



After autoclaving, let solution A and B cool to 55°C, combine and add 3 ml of filter-sterilized methanol and 1 ml of a filter-sterilized vitamin B12 solution (0,1 mg/ml). Poor the plates.