LMG media details

Last data update: 11 June 2021 14:12 CEST  

1 medium found

Name: M2GSC medium

Medium number: 417

Recipe: Casitone 10,0 g

Yeast extract 2,5 g

Glucose 2 g

Cellobiose 2 g

Soluble starch 2 g

Clarified rumen fluid 150-300 ml

KH2PO4 0,45 g

K2HPO4 0,45 g

(NH4)2SO4 0,9 g

NaCl 0,9 g

MgSO4 x 7H2O 0,09 g

CaCl2 0,09 g

Cysteine-HCl 1,0 g

NaHCO3 4 g

Reazurin 1 mg

Distilled water 1 L

Mix ingredients, except NaHCO3 and L-cysteine HCl, and adjust pH to 6. Boil until a red color develops. Dispense the medium into suitable culture vessels under a stream of H2-CO2 and seal with butyl rubber stoppers. Autoclave. Prior to inoculation, aseptically and anaerobically add the filter sterilized L-cysteine•HCl and NaHCO3 solutions.