Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | APG medium |
| Medium number: | 426 |
| Recipe: | HEPES solution 12 g (25mM) Phenol red (0,4%) 250 µl KCl 0,074 g MgSO4 x 7H2O 0,049 g CaCl2 x 2H2O 0,147 g NaCl 0,584 g pH to 8,0 with 10 M NaOH 4,4 ml Fill up with MilliQ water 1 L Autoclave and cool to room temperature Add per L: NaHCO3 (1M) 2 ml Nitrosomonas trace elements 1 ml FeNaEDTA (7,5 mM) 0,1 ml KH2PO4 (0,4 g/l) 5 ml NH4Cl (1M) 10 ml pH 8,3 at 25°C Nitrosomonas trace elements: Distilled water 987 ml HCl (25% = 7,7 M) 12,5 ml H3BO3 30 mg MnCl2 x 4H2O 100 mg CoCl2 x 6H2O 190 mg NiCl2 x 6H2O 24 mg CuCl2 x 2H2O 2 mg ZnSO4 x 7H2O 144 mg Na2MoO4 x 2H2O 36 mg Autoclave and store at 4°C FeNaEDTA solution FeNaEDTA 2753 mg Distilled water 1 L Sterilize by autoclaving or filtration. Store at 4°C. NaHCO3 (1M) 84 g NaHCO3 are dissolved in distilled water to a final volume of 1 L. Autoclave Phosphate solution Add 0,4g KH2PO4 to 1000 ml MilliQ water. Autoclave. |