Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | PYGV agar buffered with 10 mM HEPES |
| Medium number: | 427 |
| Recipe: | Mineral salt sol. ("Hutner/Cohen-Bazire") 20 ml Peptone (Bacto) 0,25 g Yeast extract 0,25 g Agar (Bacto) 15 g 10 mM HEPES Distilled water 965 ml Sterilize 20 min./121°C. After cooling to 60°C add to the medium: Glucose sol. (2.5%, sterile-filtered) 10 ml Vitamin sol. (double conc.) 5 ml Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH Mineral salt solution: Nitrilotriacetic acid (NTA) 10 g MgSO4 x 7 H2O 29,70 g CaCl2 x 2 H2O 3,34 g Na2MoO4 x 2 H2O 12,67 mg FeSO4 x 7 H2O 99 mg Metall salt sol. "44" 50 ml Distilled water 900 ml Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1 L with distilled water. Vitamin solution (double conc.): Biotin 4 mg Folic acid 4 mg Pyridoxine-HCl 20 mg Riboflavine 10 mg Thiamine-HCl x 2 H2O 10 mg Nicotinamide 10 mg D-Ca-pantothenate 10 mg Vitamin B12 0,2 mg p-Aminobenzoic acid 10 mg Distilled water 1 L Store in the dark and cold (5°C) "Metals 44": Na-EDTA 250 mg ZnSO4 x 7H2O 1095 mg FeSO4 x 7H2O 500 mg MnSO4 x H2O 154 mg CuSO4 x 5H2O 39,5 mg CoCl2 x 6H2O 20,3 mg Na2B4O7 x 10H2O 17,7 mg Distilled water 1L Dissolve the EDTA in 700 ml and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. Store at 4°C, do not autoclave |