GREAT AT SMALL THINGS

0

LMG media details

Last data update: 16 April 2021 10:30 CEST  

1 medium found

Name: Medium M13

Medium number: 436

Recipe: Peptone (Bacto) 0,25 g

Yeast extract 0,25 g

Agar (Bacto) 15 g

Hutner’s Basalt salts 20 ml

0.1M Tris-HCl, pH 7,5 50 ml

Artificial seawater 250 ml

Distilled water 660 ml

Sterilize 20 min./121°C. After cooling to 60°C add to the medium:

Glucose sol. (2.5%, sterile-filtered) 10 ml

Vitamin solution 10 ml

Adjust pH to 7.5



Artificial sea water :

NaCl 23,477 g

Na2SO4 3,917 g

MgCl2 x 6 H2O 4,981 g

CaCl2 1,102 g

NaHCO3 192 mg

KCl 664 mg

KBr 6 mg

H3BO3 26 mg

SrCl2 24 mg

NaF 3 mg

Distilled water 1 L



Vitamin solution :

Vitamin B12 0,1 mg

Biotin 2 mg

Thiamine-HCl x 2 H2O 5 mg

Ca-pantothenate 5 mg

Folic acid 2 mg

Riboflavine 5 mg

Nicotinamide 5 mg

Distilled water 1 L.

Filter sterilize. Store in the dark and cold (5°C)



Hutner’s Basalt salts:

Nitrilotriacetic acid (NTA) 10 g

MgSO4 x 7 H2O 29,70 g

CaCl2 x 2 H2O 3,34 g

Na2MoO4 x 2 H2O 12,67 mg

FeSO4 x 7 H2O 99 mg

“Metals 44" 50 ml

Distilled water up to 1 L

Dissolve NTA first by neutralizing with KOH (bout 7,3 g), then add other salts. Adjust pH to 6.8 with KOH or H2SO4. Adjust volume to 1 L with distilled water.



"Metals 44":

Na-EDTA 250 mg

ZnSO4 x 7H2O 1095 mg

FeSO4 x 7H2O 500 mg

MnSO4 x H2O 154 mg

CuSO4 x 5H2O 39,5 mg

CoCl2 x 6H2O 20,3 mg

Na2B4O7 x 10 H2O 17,7 mg

Distilled water 1L

Dissolve the EDTA in 700 ml, add the heavy metal ions one after the other and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions if necessary.