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LMG media details

Last data update: 16 June 2021 13:31 CEST  

1 medium found

Name: Methylocella silvestris medium

Medium number: 441

Recipe: MgSO4 x 7H2O 0,2 g

CaCl2 x 6H2O 0.006 g

Fe(III)Na-EDTA 0,004 g

NaNO3 0,2 g

Trace element solution 0,5 ml

Phosphate buffer 5 ml

Distilled water 1 L

Autoclave at 121°C for 15 min.

Add to the sterile medium from a filter sterilized phosphate buffer:

NaH2PO4 x 2H2O 28,71 g

Na2HPO4 x 12H2O 5,73 g

Distilled water 1 L

Trace element solution:

ZnSO4 x 7H2O 0,01 g

MnCl2 x 4H2O 0,003 g

H3BO3 0,03 g

CuCl2 x 6H2O 0,001 g

CaCl2 x 2H2O 0,02 g

Na2MoO4 x 2H2O 0,003 g

Distilled water 1 L

For solid media add 15g /l Bacto Agar.

pH of the medium should be 6,6.