Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | Methylocella silvestris medium |
| Medium number: | 441 |
| Recipe: | MgSO4 x 7H2O 0,2 g CaCl2 x 6H2O 0.006 g Fe(III)Na-EDTA 0,004 g NaNO3 0,2 g Trace element solution 0,5 ml Phosphate buffer 5 ml Distilled water 1 L Autoclave at 121°C for 15 min. Add to the sterile medium from a filter sterilized phosphate buffer: NaH2PO4 x 2H2O 28,71 g Na2HPO4 x 12H2O 5,73 g Distilled water 1 L Trace element solution: ZnSO4 x 7H2O 0,01 g MnCl2 x 4H2O 0,003 g H3BO3 0,03 g CuCl2 x 6H2O 0,001 g CaCl2 x 2H2O 0,02 g Na2MoO4 x 2H2O 0,003 g Distilled water 1 L For solid media add 15g /l Bacto Agar. pH of the medium should be 6,6. |