GREAT AT SMALL THINGS

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LMG media details

Last data update: 12 May 2021 19:50 CEST  

1 medium found

Name:

Medium number: 68

Recipe: Add 10 g glucose per liter medium 40 prior to sterilization.



Medium 40

Ground beef (fat free) 500 g

1N NaOH 25 ml

Distilled water up to 1 L

Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 minutes while stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1 liter, then add:

Casitone 30 g

Yeast extract 5 g