LMG media details

Last data update: 05 August 2022 08:32 CEST  

1 medium found

Name: Colby and Zathman medium (methanol salts medium)

Medium number: 72

Recipe: K2HPO4 1,2 g

KH2PO4 0,62 g

CaCl2 x 2H2O 34 mg

MgSO4 x 7H2O 0,2 g

NaCl 0,1 g

FeCl3 x 6H2O 1 mg

(NH4)2SO4 0,5 g

Trace element solution 1 ml (see below)

Purified agar 15 g

Distilled water up to 1 L

Adjust pH to 7,0. Autoclave at 121 °C for 15 min. Cool to 50°C. Add a filter-sterilized solution of menthanol to give a final concentration of 0,2%

Trace element solution:

CuSO4 x 5H2O 5 mg

MnSO4 x H2O 7 mg

Na2MoO4 x 2H2O 10 mg

H3BO3 10 mg

ZnSO4 x 7H2O 70 mg

CoCl2 x 6H2O 5 mg

Distilled water 1 L