GREAT AT SMALL THINGS

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LMG media details

Last data update: 26 October 2020 04:25 CET  

1 medium found

Name:

Medium number: 80

Recipe: Supplement medium 79 with 0,5 ml ethanol and 0,8 g yeast extract per liter and adjust pH to 6,8 before sterilization. Incubate in light using a tungsten lamp. If needed, add 15 g agar per liter medium.



Medium 79:

Yeast extract 0,2 g

Di-sodium succinate 1 g

Ferric citrate solution 5 ml

(0,1 g per 100 ml)

KH2PO4 0,5 g

MgSO4 x 7H2O 0,4 g

NaCl 0,4 g

NH4Cl 0,4 g

CaCl2 x 2H2O 50 mg

Trace element solution 1 ml

(see below)

Distilled water up to 1 L

pH 5,7

Trace element solution:

ZnSO4 x 7H2O 0,1 g

MnCl2 x 4H2O 30 mg

H3BO3 0,3 g

CoCl2 x 6H2O 0,2 g

CuCl2 x 2H2O 10 mg

NiCl2 x 6H2O 20 mg

Na2MoO4 x 2H2O 30 mg

Distilled water 1 L

Distribute 40 ml medium into 50 ml screw-capped bottles. Flush each bottle for 1 to 2 minutes with nitrogen gas, then close immediately with rubber septa and screw-caps. Autoclave. Sterile syringes are used to inoculate and remove the samples.