GREAT AT SMALL THINGS

0

LMG media details

Last data update: 26 October 2020 04:25 CET  

1 medium found

Name:

Medium number: 81

Recipe: Yeast extract 0,25 g

Mannitol 1,5 g

Sodium gluconate 0,5 g

KH2PO4 0,5 g

MgSO4 x 7H2O 0,4 g

NH4Cl 0,4 g

NaCl 0,4 g

CaCl2 x 2H2O 50 mg

Trace element solution 1 ml

(see medium 79)

Distilled water up to 1 L

Adjust pH to 4,9, then add:

Ferric citrate solution

(0,1 g per 100 ml) 5 ml

Biotin solution

(2 mg per 100 ml) 1 ml

p -Aminobenzoic acid

(10 mg per 100 ml) 1 ml

Sterilize in screw-capped bottles. Cool to room temperature and add 2 ml of a filter-sterilized 10% Na2S2O3SOlution per liter medium. Incubate in light using a tungsten lamp. If needed, add 15 g agar per liter medium.