Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | |
| Medium number: | 84 |
| Recipe: | To medium 80 add 25 g NaCl per liter. Incubate in light using a tungsten lamp. If needed, add 15 g agar per liter medium. Medium 80 Supplement medium 79 with 0,5 ml ethanol and 0,8 g yeast extract per liter and adjust pH to 6,8 before sterilization. Incubate in light using a tungsten lamp. If needed, add 15 g agar per liter medium. Medium 79 Yeast extract 0,2 g Di-sodium succinate 1 g Ferric citrate solution 5 ml (0,1 g per 100 ml) KH2PO4 0,5 g MgSO4 x 7H2O 0,4 g NaCl 0,4 g NH4Cl 0,4 g CaCl2 x 2H2O 50 mg Trace element solution 1 ml (see below) Distilled water up to 1 L pH 5,7 Trace element solution: ZnSO4 x 7H2O 0,1 g MnCl2 x 4H2O 30 mg H3BO3 0,3 g CoCl2 x 6H2O 0,2 g CuCl2 x 2H2O 10 mg NiCl2 x 6H2O 20 mg Na2MoO4 x 2H2O 30 mg Distilled water 1 L Distribute 40 ml medium into 50 ml screw-capped bottles. Flush each bottle for 1 to 2 minutes with nitrogen gas, then close immediately with rubber septa and screw-caps. Autoclave. Sterile syringes are used to inoculate and remove the samples. Incubate in light using a tungsten lamp. If needed, add 15 g agar per liter medium. |