Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | |
| Medium number: | 90 |
| Recipe: | Tryptone 1 g Yeast extract 1 g Salt solution 100 ml (see below) Agar 25 g Distilled water 900 ml Adjust pH to 8,2 with 1M NaOH. Autoclave 121°C, 15 minutes. Salt solution: Nitrilotriacetic acid 1 g CaSO4 x 2H2O 0,6 g MgSO4 x 7H2O 1 g NaCl 80 mg KNO3 1,03 g NaNO3 6,89 g Na2HPO4 x 12H2O 2,8 g FeCl3 x 6H2O solution (47 mg per 100 ml) 10 ml Trace element solution 10 ml (see below) Distilled water 1 L Trace element solution: H2SO4 0,5 ml MnSO4 x H2O 1,7 g ZnSO4 x 7H2O 0,5 g H3BO3 0,5 g CuSO4 x 5H2O 25 mg Na2MoO4 x 2H2O 25 mg CoCl2 x 6H2O 46 mg Distilled water 1 L |