GREAT AT SMALL THINGS

0

BCCM/MUCL culture media

Last data update: 23 November 2020 04:19 CET  

1 medium found

Short name: MMN

Name: Modified Melin-Norkrans

Medium number: 544

Recipe: malt extract - 3.0 g

D-glucose - 10.0 g

(NH4)2C4H4O6 D-ammonium tartrate -0.25 g

KH2PO4 - 0.50 g

MgSO4.7H2O - 0.15 g

CaCl2 (stock solution) - 5.0 ml

FeCl3 (stock solution) - 1.20 ml

NaCl (stock solution) - 1.0 ml

agar - 8 g

water - 1 L

pH : 5.5



Add thiamine 1.0 ml and biotine 1.0 ml (25.0 mg / L) or 0.10 ml (25.0 mg / 100 ml) after sterilization by filter on acrodisc 0.2 microns.



Stock solutions



FeCl3 (1% solution) - 1.0 g/100 ml or 1.667 g FeCl3.6H2O

NaCl - 2.50 g / 100 ml

Thiamine HCl - 0.01 g / 100 ml

CaCl2 - 1.00 g / 100 ml

Biotine - 25.0 mg / L or 25.0 mg / 100 ml