Last data update:
24 January 2024 16:39 CET
1 medium found
| Short name: | MMN |
| Name: | Modified Melin-Norkrans |
| Medium number: | 544 |
| Recipe: | malt extract - 3.0 g D-glucose - 10.0 g (NH4)2C4H4O6 D-ammonium tartrate -0.25 g KH2PO4 - 0.50 g MgSO4.7H2O - 0.15 g CaCl2 (stock solution) - 5.0 ml FeCl3 (stock solution) - 1.20 ml NaCl (stock solution) - 1.0 ml agar - 8 g water - 1 L pH : 5.5 Add thiamine 1.0 ml and biotine 1.0 ml (25.0 mg / L) or 0.10 ml (25.0 mg / 100 ml) after sterilization by filter on acrodisc 0.2 microns. Stock solutions FeCl3 (1% solution) - 1.0 g/100 ml or 1.667 g FeCl3.6H2O NaCl - 2.50 g / 100 ml Thiamine HCl - 0.01 g / 100 ml CaCl2 - 1.00 g / 100 ml Biotine - 25.0 mg / L or 25.0 mg / 100 ml |