Last data update:
24 January 2024 16:39 CET
1 medium found
Short name: | V8 then WA |
Name: | |
Medium number: | 600 |
Recipe: | 1°) Isolates were cultured on V8 pH 7.2 in Petri dishes at 25°C and exposed 12 (night) hours to UV. 2°) After 5 to 7 days growth, aerial structures were removed by scrapping the colony surface with the flamed end of a microscope slide. 3°) Discs were cut near the colony margin with a flamed cork borer, and the discs transferred in Petri dishes containing WA medium. 4°) the WA plates were in cubated in the dark at 16°C. V8 juice - 200 ml CaCO3 - 2.0 g Agar - 15 g water - 800 ml agar - 20 g water - 1 L |