Last data update:
24 January 2024 16:39 CET
1 medium found
Short name: | MSR |
Name: | Modified Strullu Romand |
Medium number: | 638 |
Recipe: | Solution 1: Macroelements MgSO4.7H2O 73,90 g/l KNO3 7,60 g/l KCl 6,50 g/l KH2PO4 0,41 g/l Solution 2: Calcium Nitrate Ca(NO3)2.4H2O 35,9 g/l Solution 3: Vitamines Panthotenate Ca 0,18 g/l Biotine (solution mère: 1 ml/500 ml (9 mg/100ml)) 0,18*10-3 g/l Nicotinic acid 0,20 g/l Pyridoxine 0,18 g/l Thiamine 0,20 g/l Cyanocobalamine 0,08 g/l Solution 4: Na Fe EDTA Na Fe EDTA 1,60 g/l Solution 5: Microelements 1. Dissolve 1,225 g of MnSO4.4H2O (35) (0,93 g of MnSO4.H2O) in 100 ml pure distilled water 2. Dissolve 0,14 g of ZnSO4.7H2O (74) in 100 ml pure distilled water 3. Dissolve 0,925 g of H3BO3 (125) in 100 ml pure distilled water 4. Mix 1+2+3 5. Weigh 1,1 g of CuSO4.5H2O (20) and dissolve in 50 ml pure distilled water 6. Pipet 5 ml to sol'n 1+2+3 7. Dissolve 0,12 g of Na2MoO4.2H2O (664) in 100 ml pure distilled water 8. When it is completely dissolved, pipet 1 ml to sol'n 1+2+3 9. Dissolve 1,7 g of (NH4)6Mo7O24.4H2O (111) in 100 ml pure distilled water 10. When it is completely dissolved, pipet 1ml in sol'n 1+2+3 11. Adjust the volume of sol'n 1+2+3 to 500 ml To prepare a 1 liter volume of solid medium 10 ml of solution 1 (macroelements) 10 ml of solution 2 (Ca(NO3)2.4H20) 5 ml of solution 4 (Na Fe EDTA) 1 ml of solution 5 (microelements) 5 ml of solution 3 (vitamins) 10 g of sucrose Dissolve completely and adjust the volume to 1 liter (H2O Type II) Adjust the pH to 5.5 Add gelling agent (3 g/l) Autoclave for 15 minutes at 121°C With powder To prepare 1L Weight 0.594g powder MSR Ca(NO3)2 0.256g Sucrose 10g Adjust the pH to 5.5 |