The OPENPichia plasmid set is a rapid and simple modular cloning system for the generation of single- or multi-gene expression constructs for Pichia pastoris (Komagataella phaffii).
This set of 70 vectors was developed by Claes et al., Nature (2024).
| You can order plasmids individually, or order the entire set at a strong discount (see price list). | Add this set to your shopping cart | ||||
| More information on the design and functionality of the set can be downloaded here: | Download the tutorial | ||||
| Plasmid information can be downloaded for the entire set: | Datasheets | Circular maps | |||
| Sequence files (GenBank) | Sequence files (EMBL) | ||||
| Accession Nr | Plasmid Designation | Part Name | Description |
| OPENPichia core vector | |||
| LMBP 12734 | pPTK081_0_Empty | Destination vector to subclone parts 1-7 | |
| Part 1 | |||
| LMBP 12735 | pPTK070_P1_ConLS | ConLS | Connector for Multigene Assembly |
| LMBP 12736 | pPTK071_P1_ConL1 | ConL1 | Connector for Multigene Assembly |
| LMBP 12737 | pPTK188_P1_ConL2 | ConL2 | Connector for Multigene Assembly |
| LMBP 12738 | pPTK189_P1_ConL3 | ConL3 | Connector for Multigene Assembly |
| LMBP 12739 | pPTK190_P1_ConL4 | ConL4 | Connector for Multigene Assembly |
| LMBP 12740 | pPTK191_P1_ConL5 | ConL5 | Connector for Multigene Assembly |
| LMBP 12741 | pPTK072_P1_ConLS' | ConLS' | Connector for Multigene Assembly |
| Part 2-4 | |||
| LMBP 13775 | pPTK109_P2-4_sfGFP | sfGFP | Fluorescent marker |
| Part 2 | |||
| LMBP 12742 | pPTK001_P2_pAOX1 | pAOX1 | Promoter |
| LMBP 12743 | pPTK034_P2_pGAP | pGAP | Promoter |
| Part 3a | |||
| LMBP 12744 | pPTK016_P3a_ScMF | ScMF | Signal peptide |
| LMBP 12745 | pPTK096_P3a_Ost1 | Ost1 | Signal peptide |
| LMBP 12746 | pPTK110_P3a_Kar2 | Kar2 | Signal peptide |
| LMBP 12747 | pPTK213_P3a_ScMFnoEAEA | ScMFnoEAEA | Signal peptide |
| Part 3b | |||
| LMBP 12748 | pPTK037_P3b_yEGFP | yEGFP | Gene of Interest |
| LMBP 12749 | pPTK146_P3b_sfGFP | sfGFP | Gene of Interest |
| Part 4 | |||
| LMBP 12750 | pPTK047_P4_*AOX1tt | *AOX1tt | Terminator |
| LMBP 12751 | pPTK052_P4_*DAS1tt | *DAS1tt | Terminator |
| Part 4a | |||
| LMBP 12752 | pPTK007_P4a_MYC-tag | MYC-tag | C-terminal tag |
| LMBP 12753 | pPTK008_P4a_HA-tag | HA-tag | C-terminal tag |
| LMBP 12754 | pPTK010_P4a_HIS-tag | HIS-tag | C-terminal tag |
| LMBP 12755 | pPTK038_P4a_yEGFP | yEGFP | Gene of Interest |
| LMBP 12756 | pPTK147_P4a_sfGFP | sfGFP | Gene of Interest |
| Part 4b | |||
| LMBP 12757 | pPTK003_P4b_AOX1tt | AOX1tt | Terminator |
| LMBP 12758 | pPTK053_P4b_DAS1tt | DAS1tt | Terminator |
| Part 5 | |||
| LMBP 12759 | pPTK073_P5_ConR1 | ConR1 | Connector for Multigene Assembly |
| LMBP 12760 | pPTK192_P5_ConR2 | ConR2 | Connector for Multigene Assembly |
| LMBP 12761 | pPTK193_P5_ConR3 | ConR3 | Connector for Multigene Assembly |
| LMBP 12762 | pPTK194_P5_ConR4 | ConR4 | Connector for Multigene Assembly |
| LMBP 12763 | pPTK195_P5_ConR5 | ConR5 | Connector for Multigene Assembly |
| LMBP 12764 | pPTK074_P5_ConRE | ConRE | Connector for Multigene Assembly |
| LMBP 12765 | pPTK075_P5_ConRE' | ConRE' | Connector for Multigene Assembly |
| Part 6 | |||
| LMBP 12766 | pPTK054_P6_Stuffer | Stuffer | Stuffer |
| LMBP 12767 | pPTK065_P6_HIS4 | HIS4 | Selection Marker |
| LMBP 12768 | pPTK200_P6_HygR | HygR | Selection Marker (Yeast) |
| LMBP 12769 | pPTK201_P6_NourR | NourR | Selection Marker (Yeast) |
| LMBP 12770 | pPTK202_P6_KanR | KanR | Selection Marker (Yeast) |
| LMBP 12771 | pPTK203_P6_ZeoR | ZeoR | Selection Marker (Yeast) |
| LMBP 12772 | pPTK042_P6_BlastR | BlastR | Selection Marker (Yeast) |
| LMBP 12773 | pPTK204_P6_Lox71-HygR-Lox66 | Lox71 - HygR - Lox66 | Floxed Selection Marker (Yeast) |
| LMBP 12774 | pPTK205_P6_Lox71-NourR-Lox66 | Lox71 - NourR - Lox66 | Floxed Selection Marker (Yeast) |
| LMBP 12775 | pPTK206_P6_Lox71-KanR-Lox66 | Lox71 - KanR- Lox66 | Floxed Selection Marker (Yeast) |
| LMBP 12776 | pPTK207_P6_Lox71-ZeoR-Lox66 | Lox71 - ZeoR- Lox66 | Floxed Selection Marker (Yeast) |
| LMBP 12777 | pPTK066_P6_Lox71-BlastR-Lox66 | Lox71 - BlastR - Lox66 | Floxed Selection Marker (Yeast) |
| Part 7 | |||
| LMBP 12778 | pPTK004_P7_Stuffer | Stuffer | Stuffer |
| LMBP 12779 | pPTK011_P7_ppARS1 | ppARS1 | Autonomously Replicating Sequence |
| LMBP 12780 | pPTK045_P7_HR1-Chr1 | HR1-Chr1 (SwaI) | Integration Site |
| LMBP 12781 | pPTK039_P7_HR1-Chr2 | HR1-Chr2 (PacI) | Integration Site |
| LMBP 12782 | pPTK040_P7_HR1-Chr3 | HR1-Chr3 (PacI) | Integration Site |
| LMBP 12783 | pPTK046_P7_HR1-Chr4 | HR1-Chr4 (SwaI) | Integration Site |
| LMBP 12784 | pPTK068_P7_I-SceIstuffer | I-SceI stuffer | Stuffer |
| Part 8 | |||
| LMBP 12785 | pPTK080_P8_AmpR | AmpR | Level 1 Destination Vector |
| LMBP 12786 | pPTK196_P8_HygR | HygR | Level 1 Destination Vector |
| LMBP 12787 | pPTK197_P8_NourR | NourR | Level 1 Destination Vector |
| LMBP 12788 | pPTK198_P8_KanR | KanR | Level 1 Destination Vector |
| LMBP 12789 | pPTK199_P8_ZeoR | ZeoR | Level 1 Destination Vector |
| LMBP 12790 | pPTK076_P8_Lox71-HygR-Lox66 | Lox71 - HygR - Lox66 | Level 1 Destination Vector |
| LMBP 12791 | pPTK077_P8_Lox71-NourR-Lox66 | Lox71 - NourR - Lox66 | Level 1 Destination Vector |
| LMBP 12792 | pPTK078_P8_Lox71-KanR-Lox66 | Lox71 - KanR - Lox66 | Level 1 Destination Vector |
| LMBP 12793 | pPTK079_P8_Lox71-ZeoR-Lox66 | Lox71 - ZeoR - Lox66 | Level 1 Destination Vector |
| Level 2 Destination Vectors | |||
| LMBP 12794 | pMC_MultiGene_NourR | NourR | destination vector for multigene assembly |
| LMBP 12795 | pMC_MultiGene_KanR | KanR | destination vector for multigene assembly |
| LMBP 12796 | pMC_MultiGene_HygR | HygR | destination vector for multigene assembly |
| LMBP 12797 | pMC_MultiGene_ZeoR | ZeoR | destination vector for multigene assembly |
| LMBP 12798 | pMC_MultiGene_BlastR | BlastR | destination vector for multigene assembly |
| Level 1 Cre plasmids | |||
| LMBP 12799 | pMC_Cre_NourR | NourR | empty vector for multigene assembly |
| LMBP 12800 | pMC_Cre_KanR | KanR | empty vector for multigene assembly |
| LMBP 12801 | pMC_Cre_HygR | HygR | empty vector for multigene assembly |
| LMBP 12802 | pMC_Cre_ZeoR | ZeoR | empty vector for multigene assembly |