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OPENPichia plasmid set

The OPENPichia plasmid set is a rapid and simple modular cloning system for the generation of single- or multi-gene expression constructs for Pichia pastoris (Komagataella phaffii).

This set of 70 vectors was developed by Vanluchene et al., 2021 (submitted).

You can order plasmids individually, or order the entire set at -40% discount.
(Discount for entire set is already included in price list.)

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Accession Nr Plasmid Designation Part Name Description
  OPENPichia core vector    
LMBP 12734 pPTK081_0_Empty   Destination vector to subclone parts 1-7
  Part 1    
LMBP 12735 pPTK070_P1_ConLS ConLS Connector for Multigene Assembly
LMBP 12736 pPTK071_P1_ConL1 ConL1 Connector for Multigene Assembly
LMBP 12737 pPTK188_P1_ConL2 ConL2 Connector for Multigene Assembly
LMBP 12738 pPTK189_P1_ConL3 ConL3 Connector for Multigene Assembly
LMBP 12739 pPTK190_P1_ConL4 ConL4 Connector for Multigene Assembly
LMBP 12740 pPTK191_P1_ConL5 ConL5 Connector for Multigene Assembly
LMBP 12741 pPTK072_P1_ConLS' ConLS' Connector for Multigene Assembly
  Part 2    
LMBP 12742 pPTK001_P2_pAOX1 pAOX1 Promoter
LMBP 12743 pPTK034_P2_pGAP pGAP Promoter
  Part 3a    
LMBP 12744 pPTK016_P3a_ScMF ScMF Signal peptide
LMBP 12745 pPTK096_P3a_Ost1 Ost1 Signal peptide
LMBP 12746 pPTK110_P3a_Kar2 Kar2 Signal peptide
LMBP 12747 pPTK213_P3a_ScMFnoEAEA ScMFnoEAEA Signal peptide
  Part 3b    
LMBP 12748 pPTK037_P3b_yEGFP yEGFP Gene of Interest
LMBP 12749 pPTK146_P3b_sfGFP sfGFP Gene of Interest
  Part 4    
LMBP 12750 pPTK047_P4_*AOX1tt *AOX1tt Terminator
LMBP 12751 pPTK052_P4_*DAS1tt *DAS1tt Terminator
  Part 4a    
LMBP 12752 pPTK007_P4a_MYC-tag MYC-tag C-terminal tag
LMBP 12753 pPTK008_P4a_HA-tag HA-tag C-terminal tag
LMBP 12754 pPTK010_P4a_HIS-tag HIS-tag C-terminal tag
LMBP 12755 pPTK038_P4a_yEGFP yEGFP Gene of Interest
LMBP 12756 pPTK147_4a_sfGFP sfGFP Gene of Interest
  Part 4b    
LMBP 12770 pPTK003_P4b_AOX1tt AOX1tt Terminator
LMBP 12758 pPTK053_P4b_DAS1tt DAS1tt Terminator
  Part 5    
LMBP 12759 pPTK073_P5_ConR1 ConR1 Connector for Multigene Assembly
LMBP 12760 pPTK192_P5_ConR2 ConR2 Connector for Multigene Assembly
LMBP 12761 pPTK193_P5_ConR3 ConR3 Connector for Multigene Assembly
LMBP 12762 pPTK194_P5_ConR4 ConR4 Connector for Multigene Assembly
LMBP 12763 pPTK195_P5_ConR5 ConR5 Connector for Multigene Assembly
LMBP 12764 pPTK074_P5_ConRE ConRE Connector for Multigene Assembly
LMBP 12765 pPTK075_P5_ConRE' ConRE' Connector for Multigene Assembly
  Part 6    
LMBP 12766 pPTK054_P6_Stuffer Stuffer Stuffer
LMBP 12767 pPTK065_P6_HIS4 HIS4 Selection Marker
LMBP 12768 pPTK200_P6_HygR HygR Selection Marker (Yeast)
LMBP 12769 pPTK201_P6_NourR NourR Selection Marker (Yeast)
LMBP 12770 pPTK202_P6_KanR KanR Selection Marker (Yeast)
LMBP 12771 pPTK203_P6_ZeoR ZeoR Selection Marker (Yeast)
LMBP 12772 pPTK042_P6_BlastR BlastR Selection Marker (Yeast)
LMBP 12773 pPTK204_P6_Lox71-HygR-Lox66 Lox71 - HygR - Lox66 Floxed Selection Marker (Yeast)
LMBP 12774 pPTK205_P6_Lox71-NourR-Lox66 Lox71 - NourR - Lox66 Floxed Selection Marker (Yeast)
LMBP 12775 pPTK206_P6_Lox71-KanR-Lox66 Lox71 -  KanR- Lox66 Floxed Selection Marker (Yeast)
LMBP 12776 pPTK207_P6_Lox71-ZeoR-Lox66 Lox71 - ZeoR- Lox66 Floxed Selection Marker (Yeast)
LMBP 12777 pPTK066_P6_Lox71-BlastR-Lox66 Lox71 - BlastR - Lox66 Floxed Selection Marker (Yeast)
  Part 7    
LMBP 12778 pPTK004_P7_Stuffer Stuffer Stuffer
LMBP 12779 pPTK011_P7_ppARS1 ppARS1 Autonomously Replicating Sequence
LMBP 12780 pPTK045_P7_HR1-Chr1 HR1-Chr1 (SwaI) Integration Site
LMBP 12781 pPTK039_P7_HR1-Chr2 HR1-Chr2 (PacI) Integration Site
LMBP 12782 pPTK040_P7_HR1-Chr3 HR1-Chr3 (PacI) Integration Site
LMBP 12783 pPTK046_P7_HR1-Chr4 HR1-Chr4 (SwaI) Integration Site
LMBP 12784 pPTK068_P7_I-SceIstuffer I-SceI stuffer Stuffer
  Part 8    
LMBP 12785 pPTK080_P8_AmpR AmpR Level 1 Destination Vector
LMBP 12786 pPTK196_P8_HygR HygR Level 1 Destination Vector
LMBP 12787 pPTK197_P8_NourR NourR Level 1 Destination Vector
LMBP 12788 pPTK198_P8_KanR KanR Level 1 Destination Vector
LMBP 12789 pPTK199_P8_ZeoR ZeoR Level 1 Destination Vector
LMBP 12790 pPTK076_P8_Lox71-HygR-Lox66 Lox71 - HygR - Lox66 Level 1 Destination Vector
LMBP 12791 pPTK077_P8_Lox71-NourR-Lox66 Lox71 - NourR - Lox66 Level 1 Destination Vector
LMBP 12792 pPTK078_P8_Lox71-KanR-Lox66 Lox71 - KanR - Lox66 Level 1 Destination Vector
LMBP 12793 pPTK079_P8_Lox71-ZeoR-Lox66 Lox71 - ZeoR - Lox66 Level 1 Destination Vector
  Level 2 Destination Vectors    
LMBP 12794 pMC_MultiGene_NourR NourR destination vector for multigene assembly
LMBP 12795 pMC_MultiGene_KanR KanR destination vector for multigene assembly
LMBP 12796 pMC_MultiGene_HygR HygR destination vector for multigene assembly
LMBP 12797 pMC_MultiGene_ZeoR ZeoR destination vector for multigene assembly
LMBP 12798 pMC_MultiGene_BlastR BlastR destination vector for multigene assembly
  Level 1 Cre plasmids    
LMBP 12799 pMC_Cre_NourR NourR empty vector for multigene assembly
LMBP 12800 pMC_Cre_KanR KanR empty vector for multigene assembly
LMBP 12801 pMC_Cre_HygR HygR empty vector for multigene assembly
LMBP 12802 pMC_Cre_ZeoR ZeoR empty vector for multigene assembly
LMBP 12803 pMC_Cre_BlastR BlastR empty vector for multigene assembly