BCCM/GeneCorner has implemented Next-Generation Sequencing as a default quality control for the authenticity of its publicly available plasmids. Since 2019, plasmids deposited in the public collection will be analysed by NGS. This way, BCCM/GeneCorner can deliver even higher quality plasmids!
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In times past, authenticity checks on GeneCorner’s plasmids were performed via restriction enzyme pattern analysis, which was carried out upon receipt of an order. Whenever this analysis was inconclusive, or showed discrepancies with the sequence obtained from the depositor, a more in-depth check was performed via Sanger sequencing. At present, plasmids that are newly deposited in the public collection, are checked for their authenticity via Next-Generation Sequencing. In case of plasmid libraries (same backbone vector, different cloned genes), a number of plasmids is selected for NGS. The bulk of the library is checked by Sanger sequencing of the insert. |
All sequence information obtained by GeneCorner is made publically accessible through our plasmid catalogue.
Plasmids that have not been sequenced (yet) only show the sequence provided by the depositor. Sanger and NGS results are made available as fasta files. Once all plasmid data have been processed to the full data set, the plasmid sequence, as modified and annotated by GeneCorner, is available for download, both in Genbank (.gb) and EMBL (.txt) format, along with a visual plasmid map (.pdf).
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