Safe deposit of cell lines, hybridomas, plasmids & genetic resources at BCCM/GeneCorner

Cell lines and hybridomas

Please complete and sign the contract and provide it to BCCM/GeneCorner.

Plasmids and other genetic resources

Please complete and sign the contract and provide it to BCCM/GeneCorner.

Technical requirements

Cell lines and hybridomas

Please send:

• minimum 20 frozen samples of the same batch containing at least 4 x 10⁶ viable cells/vial

The animal and human cell cultures or hybridomas must be checked for contaminants before submitting. BCCM/GeneCorner may refuse the deposit when cultures are thawed upon arrival. Samples must be provided in well-sealed and clearly and durably marked 1-2 ml cryotubes of ± 12 mm diameter. One or more samples will be subjected to a viability test.

Plasmids in a bacterial host

Please send:

• 3 active, freeze-dried or frozen cultures of the same batch

One or more these cultures will be subjected to a viability test and subsequently serve for the preparation of a stock of cryopreserved cells.

Plasmids as isolated material

Please send:

• 2 times minimum 20 µg plasmid DNA, in suspension, freeze-dried, frozen or precipitated in alcohol

The plasmid DNA must have a sufficient degree of purity to ensure successful transformation. The recommended bacterial host strain must be stated and - if not available at the depositary - also be furnished without the plasmid concerned. In the latter case, the storage of the appropriate host strain for the period of at least 30 years will be charged separately.

Other genetic materials

Please contact BCCM/GeneCorner

Contract termination

To end an existing contract, please fill in the termination form and provide it to BCCM/GeneCorner.