SEGA is a standardized genome engineering platform that allows for predictable expression levels and rapid interchange of functional modules. These well-characterized modules were optimized for robust performance in the context of the bacterial genome.
The subset "J23100" combines all constructs with the J23100 promoter as control element C1.
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You can order strains individually, or order a (sub)set at a strong discount (see price list). |
Add the SEGA subset "J23100" to your shopping cart | |
| Strain information can be downloaded for the entire set: | Sequence files (GenBank) |
| Full SEGA set | Subset "Untagged expression" | Subset "Building new functions" | Subset "PTrc" | Subset "PRha" | Subset "T7" |
| SEGA N° | Accession N° | E. coli strain | Locus | Upstream gadget |
C1 | C2 | Middle gadget |
Cargo | Downstream gadget |
|---|---|---|---|---|---|---|---|---|---|
| SEGA001 | LMBP 12837 | MG1655 | glmS-pstS | none | J23100 | none | none | none | 3'tetA |
| SEGA005 | LMBP 12841 | MG1655 | glmS-pstS | none | J23100 | low | BCD | gfp | tetA |
| SEGA006 | LMBP 12842 | MG1655 | glmS-pstS | none | J23100 | medium | BCD | gfp | tetA |
| SEGA007 | LMBP 12843 | MG1655 | glmS-pstS | none | J23100 | high | BCD | gfp | tetA |
| SEGA008 | LMBP 12844 | MG1655 | glmS-pstS | none | J23100 | low | Ubiquitin | gfp | tetA |
| SEGA009 | LMBP 12845 | MG1655 | glmS-pstS | none | J23100 | medium | Ubiquitin | gfp | tetA |
| SEGA010 | LMBP 12846 | MG1655 | glmS-pstS | none | J23100 | high | Ubiquitin | gfp | tetA |
| SEGA011 | LMBP 12847 | MG1655 | glmS-pstS | none | J23100 | low | TEV | gfp | tetA |
| SEGA012 | LMBP 12848 | MG1655 | glmS-pstS | none | J23100 | medium | TEV | gfp | tetA |
| SEGA013 | LMBP 12849 | MG1655 | glmS-pstS | none | J23100 | high | TEV | gfp | tetA |
| SEGA014 | LMBP 12850 | MG1655 | glmS-pstS | none | J23100 | low | PROTi | gfp | tetA |
| SEGA015 | LMBP 12851 | MG1655 | glmS-pstS | none | J23100 | medium | PROTi | gfp | tetA |
| SEGA016 | LMBP 12852 | MG1655 | glmS-pstS | none | J23100 | high | PROTi | gfp | tetA |
| SEGA017 | LMBP 12853 | MG1655 | glmS-pstS | none | J23100 | low | PelBss | sfgfp | tetA |
| SEGA018 | LMBP 12854 | MG1655 | glmS-pstS | none | J23100 | medium | PelBss | sfgfp | tetA |
| SEGA019 | LMBP 12855 | MG1655 | glmS-pstS | none | J23100 | high | PelBss | sfgfp | tetA |
| SEGA096 | LMBP 12932 | MG1655 | glmS-pstS | none | J23100 | low | BCD | none | 3'tetA |
| SEGA097 | LMBP 12933 | MG1655 | glmS-pstS | none | J23100 | high | BCD | none | 3'tetA |
| SEGA098 | LMBP 12934 | MG1655 | glmS-pstS | none | J23100 | high | BCD | gfp | 3'tetA |