SEGA is a standardized genome engineering platform that allows for predictable expression levels and rapid interchange of functional modules. These well-characterized modules were optimized for robust performance in the context of the bacterial genome.
The subset "PRha" combines all constructs with the Rha promoter as control element C1.
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You can order strains individually, or order a (sub)set at a strong discount (see price list). |
Add the SEGA subset "PRha" to your shopping cart | |
| Strain information can be downloaded for the entire set: | Sequence files (GenBank) |
| Full SEGA set | Subset "Untagged expression" | Subset "Building new functions" | Subset "J23100" | Subset "PTrc" | Subset "T7" |
| SEGA N° | Accession N° | E. coli strain | Locus | Upstream gadget |
C1 | C2 | Middle gadget |
Cargo | Downstream gadget |
|---|---|---|---|---|---|---|---|---|---|
| SEGA003 | LMBP 12839 | MG1655 | glmS-pstS | none | PrhaBAD | none | none | none | 3'tetA |
| SEGA038 | LMBP 12874 | MG1655 | glmS-pstS | none | PrhaBAD | low | BCD | gfp | tetA |
| SEGA039 | LMBP 12875 | MG1655 | glmS-pstS | none | PrhaBAD | medium | BCD | gfp | tetA |
| SEGA040 | LMBP 12876 | MG1655 | glmS-pstS | none | PrhaBAD | high | BCD | gfp | tetA |
| SEGA041 | LMBP 12877 | MG1655 | glmS-pstS | none | PrhaBAD | low | Ubiquitin | gfp | tetA |
| SEGA042 | LMBP 12878 | MG1655 | glmS-pstS | none | PrhaBAD | medium | Ubiquitin | gfp | tetA |
| SEGA043 | LMBP 12879 | MG1655 | glmS-pstS | none | PrhaBAD | high | Ubiquitin | gfp | tetA |
| SEGA044 | LMBP 12880 | MG1655 | glmS-pstS | none | PrhaBAD | low | TEV | gfp | tetA |
| SEGA045 | LMBP 12881 | MG1655 | glmS-pstS | none | PrhaBAD | medium | TEV | gfp | tetA |
| SEGA046 | LMBP 12882 | MG1655 | glmS-pstS | none | PrhaBAD | high | TEV | gfp | tetA |
| SEGA047 | LMBP 12883 | MG1655 | glmS-pstS | none | PrhaBAD | low | PROTi | gfp | tetA |
| SEGA048 | LMBP 12884 | MG1655 | glmS-pstS | none | PrhaBAD | medium | PROTi | gfp | tetA |
| SEGA049 | LMBP 12885 | MG1655 | glmS-pstS | none | PrhaBAD | high | PROTi | gfp | tetA |
| SEGA050 | LMBP 12886 | MG1655 | glmS-pstS | none | PrhaBAD | low | PelBss | sfgfp | tetA |
| SEGA051 | LMBP 12887 | MG1655 | glmS-pstS | none | PrhaBAD | medium | PelBss | sfgfp | tetA |
| SEGA052 | LMBP 12888 | MG1655 | glmS-pstS | none | PrhaBAD | high | PelBss | sfgfp | tetA |
| SEGA053 | LMBP 12889 | MG1655 | glmS-pstS | none | PrhaBAD | low | YebF | sfgfp | tetA |
| SEGA054 | LMBP 12890 | MG1655 | glmS-pstS | none | PrhaBAD | medium | YebF | sfgfp | tetA |
| SEGA055 | LMBP 12891 | MG1655 | glmS-pstS | none | PrhaBAD | high | YebF | sfgfp | tetA |
| SEGA101 | LMBP 12937 | MG1655 | glmS-pstS | none | PrhaBAD | low | BCD | none | 3'tetA |
| SEGA102 | LMBP 12938 | MG1655 | glmS-pstS | none | PrhaBAD | high | BCD | none | 3'tetA |