SEGA is a standardized genome engineering platform that allows for predictable expression levels and rapid interchange of functional modules. These well-characterized modules were optimized for robust performance in the context of the bacterial genome.
The subset "PTrc" combines all constructs with the Trc promoter as control element C1.
You can order strains individually, or order a (sub)set at a strong discount (see price list). |
Add the SEGA subset "PTrc" to your shopping cart | |
Strain information can be downloaded for the entire set: | Sequence files (GenBank) |
Full SEGA set | Subset "Untagged expression" | Subset "Building new functions" | Subset "J23100" | Subset "PRha" | Subset "T7" |
SEGA N° | Accession N° | E. coli strain | Locus | Upstream gadget |
C1 | C2 | Middle gadget |
Cargo | Downstream gadget |
---|---|---|---|---|---|---|---|---|---|
SEGA002 | LMBP 12838 | MG1655 | glmS-pstS | none | Ptrc | none | none | none | 3'tetA |
SEGA020 | LMBP 12856 | MG1655 | glmS-pstS | none | Ptrc | low | BCD | gfp | tetA |
SEGA021 | LMBP 12857 | MG1655 | glmS-pstS | none | Ptrc | medium | BCD | gfp | tetA |
SEGA022 | LMBP 12858 | MG1655 | glmS-pstS | none | Ptrc | high | BCD | gfp | tetA |
SEGA023 | LMBP 12859 | MG1655 | glmS-pstS | none | Ptrc | low | Ubiquitin | gfp | tetA |
SEGA024 | LMBP 12860 | MG1655 | glmS-pstS | none | Ptrc | medium | Ubiquitin | gfp | tetA |
SEGA025 | LMBP 12861 | MG1655 | glmS-pstS | none | Ptrc | high | Ubiquitin | gfp | tetA |
SEGA026 | LMBP 12862 | MG1655 | glmS-pstS | none | Ptrc | low | TEV | gfp | tetA |
SEGA027 | LMBP 12863 | MG1655 | glmS-pstS | none | Ptrc | medium | TEV | gfp | tetA |
SEGA028 | LMBP 12864 | MG1655 | glmS-pstS | none | Ptrc | high | TEV | gfp | tetA |
SEGA029 | LMBP 12865 | MG1655 | glmS-pstS | none | Ptrc | low | PROTi | gfp | tetA |
SEGA030 | LMBP 12866 | MG1655 | glmS-pstS | none | Ptrc | medium | PROTi | gfp | tetA |
SEGA031 | LMBP 12867 | MG1655 | glmS-pstS | none | Ptrc | high | PROTi | gfp | tetA |
SEGA032 | LMBP 12868 | MG1655 | glmS-pstS | none | Ptrc | low | PelBss | sfgfp | tetA |
SEGA033 | LMBP 12869 | MG1655 | glmS-pstS | none | Ptrc | medium | PelBss | sfgfp | tetA |
SEGA034 | LMBP 12870 | MG1655 | glmS-pstS | none | Ptrc | high | PelBss | sfgfp | tetA |
SEGA035 | LMBP 12871 | MG1655 | glmS-pstS | none | Ptrc | low | YebF | sfgfp | tetA |
SEGA036 | LMBP 12872 | MG1655 | glmS-pstS | none | Ptrc | medium | YebF | sfgfp | tetA |
SEGA037 | LMBP 12873 | MG1655 | glmS-pstS | none | Ptrc | high | YebF | sfgfp | tetA |
SEGA099 | LMBP 12935 | MG1655 | glmS-pstS | none | Ptrc | low | BCD | none | 3'tetA |
SEGA100 | LMBP 12936 | MG1655 | glmS-pstS | none | Ptrc | high | BCD | none | 3'tetA |