SEGA is a standardized genome engineering platform that allows for predictable expression levels and rapid interchange of functional modules. These well-characterized modules were optimized for robust performance in the context of the bacterial genome.
The subset "T7" combines all constructs with the T7 promoter as control element C1.
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You can order strains individually, or order a (sub)set at a strong discount (see price list). |
Add the SEGA subset "T7" to your shopping cart | |
| Strain information can be downloaded for the entire set: | Sequence files (GenBank) |
| Full SEGA set | Subset "Untagged expression" | Subset "Building new functions" | Subset "J23100" | Subset "PTrc" | Subset "PRha" |
| SEGA N° | Accession N° | E. coli strain | Locus | Upstream gadget |
C1 | C2 | Middle gadget |
Cargo | Downstream gadget |
|---|---|---|---|---|---|---|---|---|---|
| SEGA004 | LMBP 12840 | MG1655(DE3) | glmS-pstS | none | PT7 | none | none | none | 3'tetA |
| SEGA056 | LMBP 12892 | MG1655(DE3) | glmS-pstS | none | PT7 | low | BCD | gfp | tetA |
| SEGA057 | LMBP 12893 | MG1655(DE3) | glmS-pstS | none | PT7 | medium | BCD | gfp | tetA |
| SEGA058 | LMBP 12894 | MG1655(DE3) | glmS-pstS | none | PT7 | high | BCD | gfp | tetA |
| SEGA059 | LMBP 12895 | MG1655(DE3) | glmS-pstS | none | PT7 | low | Ubiquitin | gfp | tetA |
| SEGA060 | LMBP 12896 | MG1655(DE3) | glmS-pstS | none | PT7 | medium | Ubiquitin | gfp | tetA |
| SEGA061 | LMBP 12897 | MG1655(DE3) | glmS-pstS | none | PT7 | high | Ubiquitin | gfp | tetA |
| SEGA062 | LMBP 12898 | MG1655(DE3) | glmS-pstS | none | PT7 | low | TEV | gfp | tetA |
| SEGA063 | LMBP 12899 | MG1655(DE3) | glmS-pstS | none | PT7 | medium | TEV | gfp | tetA |
| SEGA064 | LMBP 12900 | MG1655(DE3) | glmS-pstS | none | PT7 | high | TEV | gfp | tetA |
| SEGA065 | LMBP 12901 | MG1655(DE3) | glmS-pstS | none | PT7 | low | PROTi | gfp | tetA |
| SEGA066 | LMBP 12902 | MG1655(DE3) | glmS-pstS | none | PT7 | medium | PROTi | gfp | tetA |
| SEGA067 | LMBP 12903 | MG1655(DE3) | glmS-pstS | none | PT7 | high | PROTi | gfp | tetA |
| SEGA068 | LMBP 12904 | MG1655(DE3) | glmS-pstS | none | PT7 | low | PelBss | sfgfp | tetA |
| SEGA069 | LMBP 12905 | MG1655(DE3) | glmS-pstS | none | PT7 | medium | PelBss | sfgfp | tetA |
| SEGA070 | LMBP 12906 | MG1655(DE3) | glmS-pstS | none | PT7 | high | PelBss | sfgfp | tetA |
| SEGA071 | LMBP 12907 | MG1655(DE3) | glmS-pstS | none | PT7 | low | YebF | sfgfp | tetA |
| SEGA072 | LMBP 12908 | MG1655(DE3) | glmS-pstS | none | PT7 | medium | YebF | sfgfp | tetA |
| SEGA073 | LMBP 12909 | MG1655(DE3) | glmS-pstS | none | PT7 | high | YebF | sfgfp | tetA |
| SEGA103 | LMBP 12939 | MG1655(DE3) | glmS-pstS | none | PT7 | low | BCD | none | 3'tetA |
| SEGA104 | LMBP 12940 | MG1655(DE3) | glmS-pstS | none | PT7 | high | BCD | none | 3'tetA |