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SEGA strains: subset "Untagged expression"

SEGA is a standardized genome engineering platform that allows for predictable expression levels and rapid interchange of functional modules. These well-characterized modules were optimized for robust performance in the context of the bacterial genome.

The subset "Untagged expression" allows for genomic modification without upstream tags.

 

You can order strains individually, or order a (sub)set at a strong discount (see price list).

  Add the SEGA subset "Untagged expression" to your shopping cart
Strain information can be downloaded for the entire set:   Sequence files (GenBank)

 

Full SEGA set   Subset "Building new functions"   Subset "J23100"   Subset "PTrc"   Subset "PRha"   Subset "T7"

 

SEGA N° Accession N° E. coli strain Locus Upstream
gadget
C1 C2 Middle
gadget
Cargo Downstream
gadget
SEGA001 LMBP 12837 MG1655 glmS-pstS none J23100 none none none 3'tetA
SEGA002 LMBP 12838 MG1655 glmS-pstS none Ptrc none none none 3'tetA
SEGA003 LMBP 12839 MG1655 glmS-pstS none PrhaBAD none none none 3'tetA
SEGA004 LMBP 12840 MG1655(DE3) glmS-pstS none PT7 none none none 3'tetA
SEGA005 LMBP 12841 MG1655 glmS-pstS none J23100 low BCD gfp tetA
SEGA006 LMBP 12842 MG1655 glmS-pstS none J23100 medium BCD gfp tetA
SEGA007 LMBP 12843 MG1655 glmS-pstS none J23100 high BCD gfp tetA
SEGA020 LMBP 12856 MG1655 glmS-pstS none Ptrc low BCD gfp tetA
SEGA021 LMBP 12857 MG1655 glmS-pstS none Ptrc medium BCD gfp tetA
SEGA022 LMBP 12858 MG1655 glmS-pstS none Ptrc high BCD gfp tetA
SEGA038 LMBP 12874 MG1655 glmS-pstS none PrhaBAD low BCD gfp tetA
SEGA039 LMBP 12875 MG1655 glmS-pstS none PrhaBAD medium BCD gfp tetA
SEGA040 LMBP 12876 MG1655 glmS-pstS none PrhaBAD high BCD gfp tetA
SEGA056 LMBP 12892 MG1655(DE3) glmS-pstS none PT7 low BCD gfp tetA
SEGA057 LMBP 12893 MG1655(DE3) glmS-pstS none PT7 medium BCD gfp tetA
SEGA058 LMBP 12894 MG1655(DE3) glmS-pstS none PT7 high BCD gfp tetA
SEGA074 LMBP 12910 MG1655 glmS-pstS none PsaI-RiboJ low BCD gfp tetA
SEGA075 LMBP 12911 MG1655 glmS-pstS none PsaI-RiboJ high BCD gfp tetA
SEGA076 LMBP 12912 MG1655 glmS-pstS none PsaI-RiboJ low BCD gfp tetA
SEGA077 LMBP 12913 MG1655 glmS-pstS none PsaI-RiboJ high BCD gfp tetA
SEGA078 LMBP 12914 MG1655 ∆galK glmS-pstS galK PrhaBAD low BCD gfp tetA
SEGA079 LMBP 12915 MG1655 ∆galK glmS-pstS galK PrhaBAD medium BCD gfp tetA
SEGA080 LMBP 12916 MG1655 ∆galK glmS-pstS galK PrhaBAD high BCD gfp tetA
SEGA096 LMBP 12932 MG1655 glmS-pstS none J23100 low BCD none 3'tetA
SEGA097 LMBP 12933 MG1655 glmS-pstS none J23100 high BCD none 3'tetA
SEGA098 LMBP 12934 MG1655 glmS-pstS none J23100 high BCD gfp 3'tetA
SEGA099 LMBP 12935 MG1655 glmS-pstS none Ptrc low BCD none 3'tetA
SEGA100 LMBP 12936 MG1655 glmS-pstS none Ptrc high BCD none 3'tetA
SEGA101 LMBP 12937 MG1655 glmS-pstS none PrhaBAD low BCD none 3'tetA
SEGA102 LMBP 12938 MG1655 glmS-pstS none PrhaBAD high BCD none 3'tetA
SEGA103 LMBP 12939 MG1655(DE3) glmS-pstS none PT7 low BCD none 3'tetA
SEGA104 LMBP 12940 MG1655(DE3) glmS-pstS none PT7 high BCD none 3'tetA