Toolkit for rapid and efficient generation of transgenic mice: RMCE-compatible plasmids + G4 ROSALUC mouse ES cells

Prof. Jody Haig's research group (IRC, VIB, Ghent, Belgium) developed a highly efficient Gateway- and recombinase-mediated cassette exchange (RMCE)-compatible system to target conditional and/or inducible constructs to the ROSA26 locus of F1 hybrid Bl6/129 ESCs, called G4 ROSALUC ES cells.
This technology allows researchers to easily create conditional and/or inducible ROSA26 alleles in a time/cost efficient manner.
Read more about this technology in Haenebalcke et al., Stem Cell Rev. 6 (2013), 774-785 [PMID: 23877658].

The plasmids of the RMCE conditional monosite system and those of the RMCE conditional and inducible multisite system are now accessible at BCCM/LMBP, as well as the G4 ROSALUC ES cells. Visit our online catalogue.


Date of publication: 
Thursday, February 6, 2014