Two-hybrid library for zebrafish available at GeneCorner

A two-hybrid plasmid library for the study of extracellular protein:protein interactions in Danio rerio, constructed by the units of Uwe Irion (Max Planck Institute for Developmental Biology, Germany) and Gavin Wright (Sanger Institute, UK), is now available at BCCM/GeneCorner.

The library currently consists of 432 plasmids in bait-prey pairs and is based on the unc5bCD4d3+4-bio vector for Avidity-based Extracellular Interaction Screening (AVEXIS) assays. The AVEXIS system allows the detection of very weak protein interactions (t1/2 < 0.1 sec) and was published by Jason Kerr and Gavin Wright (PMID: 22414956).

Click here to check the available plasmids.


Date of publication: 
Tuesday, July 7, 2020