GREAT AT SMALL THINGS

BCCM/IHEM culture media

Last data update: 11 December 2018 04:10 CET  

1 medium found

Short name: ME

Name: Malt Extract Agar

Medium number: 902

Recipe: Malt extract 20.0 g
Dextrose 20.0 g
Bacto peptone 1.0 g
MgSO4.7H2O 0.5g
KH2PO4 10.5g
Agar 20.0 g
Ultra pure water 1 l
Oligoelements solution 1 ml

Autoclave for 20 min at 120°C

Preparation of 1l oligoelements solution: 58mg H3BO3; 270mg CuCl2.2H2O; 78mg MnCl2.4H2O; 36mg (NH4)6Mo7O24.4H2O; 4.2g ZnCl2; 714mg FeCl2.4H2O solved in 3ml HCl 1N; 1l Ultra pure water; Sterilize on 0,22m filter