Last data update:
24 January 2024 16:39 CET
1 medium found
Name: | BCYE alpha agar medium |
Medium number: | 1003 |
Recipe: | Yeast extract 10 g Activated charcoal (Norit SG) 2 g ACES buffer (Sigma) 10 g Alpha-ketoglutarate potassium 1 g Agar 17 g Hydrate ACES in 500 ml distilled water at 50°C, then mix with a solution containing 40 ml of 1 N KOH in 440 ml distilled water. Use the mixture to hydrate the other ingredients. Autoclave for 15 minutes. After sterilization, cool to 50°C, and add the following filter-sterilized solution: Cysteine hydrochloride 0,4 g Fe4(P2O7)3 0,25 g Distilled water 20 ml The final pH of the medium should be 6,9. |