Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | Modified Biebl & Pfennings medium |
| Medium number: | 1004 |
| Recipe: | KH2PO4 0,5g CaCl2 x 2H2O 50 mg MgSO4 x 7H2O 0,2 g NH4Cl 0,4 g NaCl 0,4 g Sodium pyruvate 3 g Ferric citrate solution 1 ml (0,1 g per 100 ml) Micronutrient solution SL-7 1 ml (see below) Na2S (1M) 1 ml NaHCO3 (10%) 10 ml Distilled water up to 1 L Na2S and NaHCO3 should be added to the media separately only after autoclaving. pH should be adjusted to 7.0 - 7.5 using 1N HCl/1N NaOH Micronutrient solution SL-7: HCl 25% 1 ml ZnCl2 70 mg MnCl2 x 4H2O 0,1 g H3BO3 60 mg CoCl2 x 6H2O 0,2 g NiCl2 x 6H2O 20 mg Na2MoO4 x 2H2O 40 mg CuCl2 x 2H2O 20 mg Distilled water 1 L |